JIS Z 2801 test used for detection of Antimicrobial Activity of Plastics

JIS Z 2801 is an antibacterial surface method that tests antibacterial activity and antibacterial effect. The JIS Z 2801 method tests plastics, metals, ceramics, and other antibacterial surfaces to suppress or kill the growth of microorganisms. This procedure is susceptible to antibacterial activity and is used in many real applications everywhere, from hospitals / clinical settings to home consumer companies concerned about the materials’ ability to enable bacterial growth.

The JIS Z 2801 method is the most commonly selected test and has become the industry standard for antibacterial performance on hard surfaces in the United States. Below is an overview of the JIS Z 2801 test method and some of its strengths and weaknesses of this test. The JIS Z 2801 test method is designed to quantitatively test the ability of a hard surface to suppress microbial growth and kill microbial organisms during a 24-hour contact period.

The JIS Z 2801 procedure is adopted as the ISO 22196 procedure of the International Organization for Standardization (ISO).

Japanese Industrial Standards (JIS) Z 2801 is a generally required quantitative antibacterial test for plastics, foams, and fibers and is in harmony with ISO 22196. The JISZ2801 standard method tests hard surfaces and plastics to measure the growth that can occur after that. Two common bacteria, E. coli and Staphylococcus aureus, are inoculated. The standard test is 24 hours.

The JIS Z 2801 is one of the most requested, fast, and reliable antibacterial surface tests to measure antibacterial activity and antibacterial effect.

Durability testing in combination with JIS Z 2801 can provide important information about the antibacterial performance when exposed to various environmental conditions such as heat, humidity, and UV light.

As with all antibacterial tests, we recommend that you first follow the requirements of your end customer and their respective regulators when choosing a test method.

Advantages of the JIS Z 2801 test method:

One of the original antibacterial test methods. JIS Z 2801 was developed for materials and products entering the Japanese market. The JIS standard is a highly reliable and globally recognized antibacterial test. JIS standards are unified in a wide range of needs.

The harmonized version of JIS Z 2801, ISO 22196, is almost the same as JIS Z 2801.


The JIS (Japanese Industrial Standards) Z 2801 method was designed to test plastics’ antibacterial activity/effectiveness.

In 2007, the JIS Z2801 method was internationally standardized as ISO22196.

JIS Z 2801 sample requirements:

  • The size of the sample should be 5 x 5 cm.
  • At least six blank samples are required for each measurement setup.
  • A sample with at least three antibiotics is required for a single measurement.
  • The surface of the sample should be smooth.
  • Paints / colors / lacquers / coatings can be delivered in liquid form. Paint the LENETA test plate (as a support) for paint / color / lacquer / coating.
  • Pack the sample and label the sample lot, serial number, or information you want to convey about the content accordingly.
  • Do not mark the sample with a felt-tip pen or marker.
  • Those colors are easy to implement and often result in improperness. This also applies to oil-based markers. If you need to label the sample, use a pencil.

JIS Z 2801 test method:

-Test organisms are extracted directly from the agar plate and diluted with buffer

-Microbial concentrations are standardized by diluting with nutrient broth

-Inoculate the control and test surfaces with 0.4 ml of bacterial suspension three times.

-The inoculum is covered with a film to prevent evaporation of the suspension and ensure close contact with the sample’s surface.

-Immediately after injection, the bacteria in the reference sample are isolated from the sample surface. The number of viable bacteria (t0 value) is determined by elution followed by dilution and plating.

-Other samples (t24) are incubated in a humid environment for 24 hours.

-After incubation, microbial concentration is determined by elution followed by dilution and plating

-Activity/efficacy is calculated by comparing the concentration of blanks after 24 hours with the concentration of bacteria after 24 hours

Strengths of the JIS Z 2801 test method:

-Reproducibility and reproducibility are satisfactory (ISO shows at least 60% variation.

S Z 2801 specifies how to measure the antibacterial activity of our antibacterial/antibacterial treated plastic products such as Si-Quat products.

It is not designed to assess the effects and spread of bacteria on plastics without antibacterial treatment but is specially designed to test Si-Quat and Clean N Coat products. ISO 846 describes tests to assess the effects and spread of bacteria in plastics. This is different from the one covered by JIS Z 2801. For some tests, it is recommended to test the untreated plastic to create a control group. Antibacterial treatments such as biodeterioration and odor prevention are side effects of our Si-Quat functional coating but are not covered by the standard.

Test microorganisms are usually prepared by growth in a liquid medium. This method identifies two typical organisms, Staphylococcus aureus and Escherichia coli. These are standard and are also used to test Si-Quat functional coatings in AFFIX labs.

The microbial test suspension is standardized by diluting a nutrient solution that allows the bacteria to grow during the test.

Inoculate the control and test surfaces with microorganisms, and then cover the microbial inoculum with a thin, sterile cover. Covering the inoculum material improves the spread of the inoculum material and prevents vaporization. It also helps ensure close contact with the antibacterial surface required for mechanical killing, as well as active Si-Quat molecules.

Microbial concentration is established by elution at the beginning of the test, followed by dilution and plating.

Controls are performed to ensure that the neutralization/elution method effectively neutralizes the antibacterial agent on the antibacterial surface under test.

All samples and control groups are incubated and kept quiet for 24 hours in a humid environment.

After culturing, microbial concentration is measured. The initial concentration and the reduction of microorganisms on the control surface are calculated.


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